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Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids
This content is Free Access.
Chapters
- 00:05Title
- 00:43Creating Cerebral Organoids from iPSC / hESC 2D Culture (Day 0)
- 03:30Cerebral Organoid Maintenance (Day 2)
- 04:37Cerebral Organoid Maintenance (Day 3)
- 05:14Cerebral Organoid Maintenance (Day 4+)
- 06:13Organoid Infection with ZIKV (~Day 24)
- 07:45Results: Characterization of ZIKB-infected Organic Degradation
- 08:47Conclusion
This protocol describes a technique used to model Zika virus infection of the developing human brain. Using wildtype or engineered stem cell lines, researchers may use this technique to uncover the various mechanisms or treatments that may affect early brain infection and resulting microcephaly in Zika virus-infected embryos.
