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Journal / Immunology and Infection / Sobreexpresión y Purificación de Humanos
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Immunology and Infection

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JoVE Journal Immunology and Infection
Sobreexpresión y Purificación de Humanos
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Sobreexpresión y Purificación de Humanos

Article DOI: 10.3791/56430-v • 07:35 min • August 3rd, 2017
August 3rd, 2017 •
*1, *1, 2, 2, 1,3, 1,3, 2, 2,4, 2
1Sackler Faculty of Medicine, Tel Aviv University, 2Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, 3Department of Ophthalmology, Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University, 4Tel Aviv Sourasky Medical Center, affiliated to the Sackler Faculty of Medicine, Tel Aviv University
* These authors contributed equally

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Un protocolo simple para la sobreexpresión y purificación de la cis- preniltransferasa humana optimizada con codones, en condiciones no desnaturalizantes, de Escherichia Coli , junto con un ensayo de actividad enzimática. Este protocolo puede generalizarse para la producción de otras proteínas de cis- preniltransferasa en cantidad y calidad adecuadas para estudios mecánicos.

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Immunology Número 126 optimización de codones sobreexpresión heteróloga purificación de proteínas preniltransferasa dehidrodolichil difosfato sintasa poliprenil
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