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Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

DOI:
10.3791/56459-v

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09:14 min

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October 19, 2017

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, , , ,
1Monash Lung and Sleep,Monash Medical Centre, 2Monash University

Chapters

  • 00:05Title
  • 00:46Collect, Fluorescently Label, and Image Bronchoalveolar Lavage Macrophages
  • 02:50Preparation/Microscopy of Lung Tissue Samples
  • 05:35Three-dimensional Imaging of METs
  • 06:54Results: Macrophage Extracellular Trap Visualization
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

Macrophage extracellular traps are a newly described entity. This article will concentrate on confocal microscopy methods and how they are visualized in vitro and in vivo from lung samples.

Tags

Keywords: Macrophage Extracellular TrapsConfocal MicroscopyInflammationBronchoalveolar LavageTrypan BlueHemocytometerParaformaldehyde-periodate-lysine FixativeTWEEN 20Chicken SerumBSAPrimary AntibodiesSecondary AntibodiesDAPIFFPE SamplesAntigen RetrievalPressure CookerTris-EDTA

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