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Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
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Developmental Biology
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JoVE Journal Developmental Biology
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

DOI:
10.3791/56498-v

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07:40 min

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December 08, 2017

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,
1BioMEMS Resource Center, Department of Surgery,Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital

Chapters

  • 00:05Title
  • 01:06Orientation of Zebrafish on Divot Arrays and Microstructured Channels
  • 02:39Microinjection of Zebrafish
  • 04:20Screening of Embryos Using the PDMS Imaging Device
  • 05:45Results: Microinjection and Imagingof Zebrafish Larvae Using Microstructured Devices
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

Microinjection of zebrafish embryos and larvae is a crucial but challenging technique used in many zebrafish models. Here, we present a range of microscale tools to aid in the stabilization and orientation of zebrafish for both microinjection and imaging.

Tags

Keywords: MicroinjectionZebrafish LarvaeMicrostructured DevicesPDMSMicroarrayMicrofeaturesImmobilizationOrientationImagingSingle-cell ResolutionDisease Models

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