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Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

Fabrication of Custom Agarose Wells for Cell Seeding and Tissue Ring Self-assembly Using 3D-Printed Molds

DOI:
10.3791/56618-v

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08:16 min

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April 02, 2018

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, , , ,
1Biomedical Engineering,Worcester Polytechnic Institute, 2Gladstone Institute for Cardiovascular Disease

Chapters

  • 00:05Title
  • 00:363D-printing the Mold and Casting the Polydimethylsiloxone (PDMS) Negative
  • 02:41Agarose Mold Fabrication
  • 04:01Seeding Tissue Rings with Rat Aortic Smooth Muscle Cells (RaSMCs)
  • 06:56Results: Self-assembled Tissue Rings
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a platform for fabricating self-assembled tissue rings in variable sizes using a customized 3D-printed plastic mold. PDMS negatives are cured in the 3D-printed mold; then agarose is cast in the cured PDMS negatives. Cells are seeded into the resulting agarose wells where they aggregate into tissue rings.

Tags

Keywords: 3D-printed MoldsAgarose WellsCell SeedingPDMSComputer-aided Design

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