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Biology
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体外
sumo 法研究相扑 E3 连接活动
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Biology
In Vitro
SUMOylation Assay to Study SUMO E3 Ligase Activity
Please note that all translations are automatically generated.
Click here for the English version.
体外
sumo 法研究相扑 E3 连接活动
DOI:
10.3791/56629-v
•
09:45 min
•
January 29, 2018
•
Wan-Shan Yang
,
Mel Campbell
,
Hsing-Jien Kung
3,4,5
,
Pei-Ching Chang
6
1
Institute of Microbiology and Immunology
,
National Yang-Ming University
,
2
UC Davis Cancer Center
,
University of California, Davis
,
3
Department of Biochemistry and Molecular Medicine
,
University of California, Davis
,
4
Institute for Translational Medicine, College of Medical Science and Technology
,
Taipei Medical University
,
5
Division of Molecular and Genomic Medicine
,
National Health Research Institutes
,
6
Center for Infectious Disease and Cancer Research
,
Kaohsiung Medical University
Chapters
00:05
Title
00:39
Purification of K-bZIP
03:26
SUMOylation Assay
07:15
Results: Use of
In Vitro
SUMOylation Assay to Study the Role of SUMO E3 Ligase in p53 SUMOylation Using Optimal Ubc9 Enzyme Concentration
08:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
不像泛素酶, 很少有 E3 相扑酶被发现。这一修改的
体外
sumo 协议能够识别新的相扑 E3 酶由一个
体外
重建化验。
Tags
In Vitro SUMOylation
SUMO E3 Ligase Activity
SUMO E3 Ligase Identification
SUMO Isoform Specificity
Protein Purification
Magnetic Bead Pulldown
SDS-PAGE
Protein Quantification
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