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Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
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Developmental Biology
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JoVE Journal Developmental Biology
Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice

DOI:
10.3791/56670-v

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12:06 min

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November 18, 2017

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1School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine,Washington State University

Chapters

  • 00:00Title
  • 02:02Mice, Mating, and Assessing Copulatory Plug
  • 03:25Preparation Before Tissue Collection
  • 04:40Tissue Collection
  • 07:11Measurement of Semen Liquefaction from the Uterine Content Collection
  • 09:25Results
  • 10:02Example of Downstream Application: Video Imaging of Sperm Motility
  • 11:13Conclusion

Summary

Automatic Translation

Automatic Translation

Semen liquefaction is required for sperm to be liberated from the seminal gel. This study provides the procedures for collecting semen from the female reproductive tract post-coitus, and measuring semen liquefaction time.

Tags

Keywords: Post-mating SemenSemen LiquefactionMiceFemale Reproductive TractSemen CollectionSperm MotilityProstate Specific Antigen (PSA)InfertilityVaginal PlugLeibovitz’s L-15 MediaFetal Bovine Serum

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