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JoVE Journal Neuroscience
En enkel cellebasert Immunofluorescence analysen å oppdage Autoantibody mot N-Methyl-D-Aspartate (NMDA) reseptoren i blod
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En enkel cellebasert Immunofluorescence analysen å oppdage Autoantibody mot N-Methyl-D-Aspartate (NMDA) reseptoren i blod

Article DOI: 10.3791/56676-v • 07:20 min • January 9th, 2018
January 9th, 2018 •
1,2, 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University

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Vi uttrykte ectopically NR1 delenhet i NMDA reseptor merket med grønne fluorescerende protein i menneskelige embryonale celler (HEK293) som antigen å oppdage autoantistoffer mot NMDA reseptor i blodet av pasienter mistenkt med autoimmune encefalitt. Denne enkle metoden kan være passende for screening formål i klinisk innstillinger.

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Nevrovitenskap problemet 131 Anti-NMDA reseptor autoantibody NR1 delenhet grønne fluorescerende protein autoimmune encefalitt cellen-basert immunofluorescence analysen differensialdiagnose nevropsykiatriske symptomer
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