Journal
/
Immunology and Infection
/
Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

DOI:
10.3791/56689-v

•

10:35 min

•

December 26, 2017

•

,
1British Columbia Children’s Hospital Research Institute,University of British Columbia

Chapters

  • 00:05Title
  • 00:44Bone Marrow Macrophage Derivation and Activation with Antibodies: In Vitro Testing
  • 05:34Harvesting Peritoneal Macrophages after an IVIg + LPS Challenge: In Vitro Testing
  • 08:38Results: BMDM Reaction to IVIg + LPS Treatment
  • 10:06Conclusion

Summary

Automatic Translation

Automatic Translation

Antibody-based drugs have revolutionized treatment for inflammatory diseases. In addition to having direct effects on specific targets, antibodies can activate macrophages to become anti-inflammatory. This protocol describes how anti-inflammatory macrophage activation can be assessed in vitro, using mouse bone marrow macrophages, and in vivo, using peritoneal macrophages.

Tags

Keywords: Antibody-based DrugsMurineBone MarrowPeritoneal MacrophagesAnti-inflammatoryIL-10ImmunologyPrimary MacrophagesBone Marrow In VitroPeritoneal Cavity In VivoEuthanized MouseBone Marrow IsolationBone Marrow CultureMCSF

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56689?article_type=v

Murine Model of CD40-activation of B cells
check_url/56689?article_type=v

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
check_url/56689?article_type=v

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
check_url/56689?article_type=v

Bone Marrow-derived Macrophage Production
check_url/56689?article_type=v

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
check_url/56689?article_type=v

Tracking Mouse Bone Marrow Monocytes In Vivo
check_url/56689?article_type=v

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
check_url/56689?article_type=v

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
check_url/56689?article_type=v

Isolation and Activation of Murine Lymphocytes
check_url/56689?article_type=v

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Read Article