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Trans-indre celle masse injektion af stamceller fra embryoner fører til højere kimærisme

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JoVE Journal Biology

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-indre celle masse injektion af stamceller fra embryoner fører til højere kimærisme

DOI:

10.3791/56955-v

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05:53 min

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May 29, 2018

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Gregory J. Scott, Artiom Gruzdev, Thomas B. Hagler, Manas K. Ray

¹Knock Out Mouse Core,NIEHS/NIH

Chapters

00:05Title
00:44Embryo Collection
02:01Embryo Injection and Transfer
04:26Results: Representative Post-embryonic Stem (ES) Cell Transfer Pregnancy, Survival, and Chimersim Rates
04:59Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol for at øge chimera produktion uden brug af nyt udstyr. En simpel orientering ændring af embryo til injektion kan øge antallet af embryoner produceret og potentielt reducere tidslinjen til kønscelleoverførsel transmission.

Trans-indre celle masse injektion af stamceller fra embryoner fører til højere kimærisme

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Chapters

Summary

Automatic Translation

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