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Trans-indre cellen masse injeksjon av embryonale stamceller fører til høyere Chimerism priser
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JoVE Journal Biology
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-indre cellen masse injeksjon av embryonale stamceller fører til høyere Chimerism priser

DOI:
10.3791/56955-v

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05:53 min

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May 29, 2018

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, , ,
1Knock Out Mouse Core,NIEHS/NIH

Chapters

  • 00:05Title
  • 00:44Embryo Collection
  • 02:01Embryo Injection and Transfer
  • 04:26Results: Representative Post-embryonic Stem (ES) Cell Transfer Pregnancy, Survival, and Chimersim Rates
  • 04:59Conclusion

Summary

Automatic Translation

Automatic Translation

Her presenterer vi en protokoll for å øke chimera produksjon uten bruk av nytt utstyr. En enkel retning endring av embryoet for injeksjon kan øke antall embryo produsert, og potensielt redusere tidslinjen germline overføring.

Tags

Keywords: Embryonic Stem CellsChimeraTrans-inner Cell Mass InjectionBlastocystMicroinjectionCell LineCell CultureBiologistGraduate StudentMicroscopyLaboratory Techniques

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