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JoVE Journal
Medicine

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Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)
 

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

Article DOI: 10.3791/57006 09:47 min September 13th, 2018
September 13th, 2018

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Summary

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.

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Tags

Standardized Measurement Nasal Membrane Transepithelial Potential Difference NPD CFTR Function Epithelial Sodium Channel Airway Epithelium Cystic Fibrosis Genetic Disorders Hyperactive Epithelial Sodium Channel Function Thao Nguyen Aur Voltage Change Ion Channel Activity Nasal Epithelium Subcutaneous Compartment Inferior Nasal Turbinate Amiloride Inhibitor
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