Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

George M. Solomon; Inez Bronsveld; Kathryn Hayes; Michael Wilschanski; Paola Melotti; Steven M. Rowe; Isabelle Sermet-Gaudelus

doi:10.3791/57006

Journal

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Medicine

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Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

16,382 Views

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09:47 min

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September 13, 2018

DOI:

10.3791/57006-v

DOI:

10.3791/57006-v

•

09:47 min

September 13, 2018

•

16351 Views

George M. Solomon¹, Inez Bronsveld², Kathryn Hayes³, Michael Wilschanski⁴, Paola Melotti⁵, Steven M. Rowe¹, Isabelle Sermet-Gaudelus^6,7

¹Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center,University of Alabama at Birmingham ²Department of Pulmonology and Tuberculosis,University Medical Center Utrecht ³Center for Experimental Medicine,Queens University, Northern Ireland ⁴Hadassah Hebrew University Medical Center, Jerusalem ⁵Centro Fibrosi Cistica,Azienda Ospedaliera Universitaria Integrata ⁶Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose,Hôpital Necker Enfants Malades ⁷INSERM U 1151,Institut Necker Enfants Malades

Summary

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.