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Chapters
- 00:04Introduction
- 00:55Stripping the Muscle Layer and Preparing the Intestinal Sheet
- 02:12Mounting Intestinal Preparations in Ussing Chambers
- 03:05Measurement of Dilution Potential (Open Circuit Conditions)
- 04:07Transepithelial Electrical Conductance and Baseline Isc (Short-Circuit Conditions)
- 04:55Results: Transepithelial Electrical Conductance and Dilution Potential in Cldn15-/- Mice
- 06:12Conclusion
Intestinal epithelium confers not only nutrient absorption but protection against noxious substances. The apical-most epithelial intercellular junction, i.e., the tight junction, regulates paracellular solute and ion permeability. Here, a protocol for the preparation of mucosal sheets and assessment of the ion selectivity of tight junctions using Ussing chamber technique is described.
