Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.

Primary Human Nasal Epithelial Cells

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

Article DOI: 10.3791/63409-v 08:35 min April 22nd, 2022
April 22nd, 2022



Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.



Primary Nasal Epithelial Cells Biobanking Precision Medicine Culture Conditions Patient-derived Cultures Personalized Medicine Studies CFTR Activity Cystic Fibrosis Therapy Nasal Epithelial Or HNE Cells Amplification Medium 0.25% Trypsin Cell Detachment
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter