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Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine
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Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

DOI:
10.3791/63409-v

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08:35 min

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April 22, 2022

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, , , , , ,
1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées,Assistance Publique Hôpitaux de Paris

Chapters

  • 00:04Introduction
  • 00:56Amplification and Passaging of Human Nasal Epithelial (HNE) Cells
  • 02:26Cryopreservation of Primary Nasal Epithelial Cells
  • 03:06Thawing Frozen Amplified HNE Cells
  • 04:38Differentiation of HNE Cells at the Air-Liquid Interface
  • 05:23Results: Analysis of Electrophysiological Properties of Differentiated HNE Cells and CFTR-Related Chloride Secretion Correction
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.

Tags

Primary Nasal Epithelial CellsBiobankingPrecision MedicineLung EpitheliumPatient-derived CulturesPersonalized MedicineCFTR ActivityCystic Fibrosis TherapyAmplification MediumCryopreservationCell CultureCell CountingFreezing MediumCryofreezingNitrogen Storage

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