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Primary Human Nasal Epithelial Cells

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

Article DOI: 10.3791/63409-v
April 22nd, 2022


Summary April 22nd, 2022

Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.


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