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Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine
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Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine

English

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2,578 Views

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08:35 min

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April 22, 2022

DOI:

10.3791/63409-v

DOI:
10.3791/63409-v

•

08:35 min
April 22, 2022

•

8 Views
, , , , , ,
1Institut Necker Enfants Malades, 2Université de Paris, 3Centre de Référence Maladies Rares Mucoviscidose et Maladies apparentées,Assistance Publique Hôpitaux de Paris

Summary

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Here we describe the isolation, amplification, and differentiation of primary human nasal epithelial (HNE) cells at the air-liquid interface and a biobanking protocol allowing to successfully freeze and then thaw amplified HNE. The protocol analyzes electrophysiological properties of differentiated HNE cells and CFTR-related chloride secretion correction upon different modulator treatments.

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Cite this Article

Kelly, M., Dreano, E., Hatton, A., Lepissier, A., Golec, A., Sermet-Gaudelus, I., Pranke, I. Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine. J. Vis. Exp. (182), e63409, doi:10.3791/63409 (2022).

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