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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af dendritiske celler fra det menneskelige kvindelige forplantningsorganerne for Phenotypical og funktionelle studier
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Isolering af dendritiske celler fra det menneskelige kvindelige forplantningsorganerne for Phenotypical og funktionelle studier

Article DOI: 10.3791/57100-v • 12:32 min • March 13th, 2018
March 13th, 2018 •
1, 1, 1, 1
1Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth

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Vi beskriver her en metode til at isolere og rense dendritiske celler fra forskellige anatomiske rum i den menneskelige kvindelige forplantningsorganerne for evalueringen af deres phenotypical og funktionelle egenskaber. Denne metode kan tilpasses til at isolere andre immunceller eller dendritiske celler fra andre slimhinden væv.

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Immunologi og infektion sag 133 dendritiske celler kønsorganer slimhinde endometriet endocervix ectocervix kvinder allogene stimulation T-celle spredning CD1a CD1c monocyt-afledte celler
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