Dead Cell Removal
Dead Cell Removal - Related Videos
10:47
Quantification of Proliferative and Dead Cells in Enteroids
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Cited by 2 •
2020
The presented protocol uses flow cytometry to quantify the number of proliferating and dead cells in cultured mouse enteroids. This method is helpful to evaluate the effects of drug treatment on organoid proliferation and survival.
09:48
In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages
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Cited by 7 •
2021
Neurodegenerative diseases are associated with dysregulated microglia functions. This article outlines an in vitro assay of phagocytosis of neuroblastoma cells by iPSC-macrophages. Quantitative microscopy readouts are described for both live-cell time-lapse imaging and fixed-cell high-content imaging.
05:07
Alternative Method of Removing Otoliths from Sturgeon
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Cited by 3 •
2016
The goal of the protocol is to show an effective method to extract the otoliths from sturgeon carcasses.
03:41
C. elegans Blastomere Dissection: A Method to Remove the Eggshell and Dissociate Embryonic Cells
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2023
This video introduces a method of isolating C. elegans blastomeres from early embryos. The resulting cells are suitable for cell culture or ex vivo experiments.
07:12
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
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Cited by 3 •
2012
Despite ongoing efforts to transition cultures to feeder-free conditions, the derivation and culture of human embryonic stem cells (hESC) remain largely dependent on co-cultures with mouse embryonic feeders (MEFs). Here, we show a novel methodology for rapidly removing feeders from hESC cultures prior to experimentation.
FAQs
Recognition of dying cells depends on coordinated signaling rather than debris accumulation alone. Phagocytes detect “find-me” signals that help locate dying cells and “eat-me” signals that support their uptake. After engulfment, the material enters phagolysosomes, where it is digested. Studying these stages helps distinguish failures in detection, internalization, or intracellular processing.
Macrophages are among the phagocytes that recognize and engulf dying cells. Their activity connects the identification of cellular remains with digestion inside phagolysosomes, allowing removal to occur within tissues. In immunology research, examining macrophage behavior can therefore clarify how local clearance contributes to tissue homeostasis and influences the surrounding inflammatory response.
Efficient clearance limits the persistence of cellular remains that could disrupt tissue function or provoke harmful inflammation. When removal is defective, inflammation may continue, tissue repair may be impaired, and host responses can become altered. These consequences make clearance mechanisms relevant to studies of inflammatory disease, autoimmunity, and infection-related tissue damage.
Defective clearance can alter host defense by changing how tissues manage cellular remains during infection. It may also prolong inflammation or impair repair, affecting the tissue environment in which immune responses unfold. Researchers can use this connection to investigate why infected tissues develop different inflammatory outcomes and how clearance relates to pathogen-associated immune responses.
A useful analysis follows the process from signal recognition through engulfment and intracellular digestion. Researchers can examine whether phagocytes respond to “find-me” signals, recognize “eat-me” signals, internalize the dying material, and process it in phagolysosomes. Separating these stages helps identify where clearance fails and links that failure to tissue or immune outcomes.
These studies can connect cellular clearance with tissue homeostasis, inflammatory regulation, and repair. In immunology and infection research, they also help explain how altered removal affects autoimmunity, inflammatory disease, and host defense. The resulting framework supports comparisons between effective and defective clearance and clarifies how dying-cell handling shapes broader immune behavior.