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Immunology and Infection
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Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
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Immunology and Infection
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JoVE Journal Immunology and Infection
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

DOI:
10.3791/57191-v

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07:06 min

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February 06, 2018

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, , ,
1Department of Anatomy,Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science,Yonsei University College of Medicine, 3Department of Surgery,Yonsei University College of Medicine

Chapters

  • 00:05Title
  • 00:28Surgical Process
  • 03:06Intravital Imaging of the Liver with Two-photon MIcroscopy
  • 04:51Results: Two-photon Intravital Imaging in LPS-treated Mouse Liver
  • 06:03Conclusion

Summary

Automatic Translation

Automatic Translation

We established a novel surgical protocol for two-photon imaging of live mice liver with minimal invasion. With this technique, we identified the detailed structure of the liver during lipopolysaccharide-induced endotoxemia. We anticipate that this method may be utilized to determine the effectiveness of various reagents treatment to hepatic leukocyte migration.

Tags

Keywords: Two-photon Intravital ImagingLeukocytesImmune ResponseLipopolysaccharideMouse LiverSeptic ShockLiver SurgeryAnesthesiaTexas Red DextranPovidone IodineAbdominal CavityLiver ExposureIntravital ImagingLaser SettingsWater Immersion Objective LensTemperature Control

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