Septic Shock Model
Septic Shock Model - Related Videos
16:31
Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
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Cited by 24 •
2011
The Hemorrhagic Shock model has been a reliable and reproducible resource facilitating the identification and understanding of signaling cascades associated with inflammation and end-organ damage after trauma. This article provides a step-by-step description of surgical and mechanical aspects associated with the Hemorrhagic Shock experimental procedure in mice.
06:11
Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring
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Cited by 19 •
2017
Uncontrolled hemorrhage, an important cause of mortality among trauma patients, can be modeled using a standard liver laceration in a murine model. This model results in consistent blood loss, survival, and allows for testing hemostatic agents. This article provides the step-by-step process to perform this valuable model.
07:15
In vivo Macrophage Imaging Using MR Targeted Contrast Agent for Longitudinal Evaluation of Septic Arthritis
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Cited by 2 •
2013
We demonstrate how to perform macrophage MR imaging using ultrasmall superparamagnetic contrast agent (USPIO) in septic arthritis, allowing an initial and longitudinal in vivo non-invasive evaluation of macrophages infiltration and an assessment of therapy efficacy.
09:48
Drosophila Adult Olfactory Shock Learning
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Cited by 36 •
2014
The method to measure adult Drosophila associative memory is described. The assay is based on the ability of the fly to associate an odor presented with a negative reinforcer (electric shock) and then recall this information at a later time, allowing memory to be measured.
11:01
Bacterial Transformation: The Heat Shock Method
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2023
Transformation is the process that occurs when a cell ingests foreign DNA from its surroundings. Transformation can occur in nature in certain types of bacteria. In molecular biology, transformation is artificially reproduced in the lab via the creation of pores in bacterial cell membranes. Bacterial cells that are able to take up DNA from the environment are called competent cells. In the laboratory, bacterial cells can be made competent and DNA subsequently introduced by a procedure called...
FAQs
Microbial products or infectious agents initiate an uncontrolled immune response that releases cytokines, signaling proteins involved in inflammation. This response can impair endothelial function, promote vasodilation, and increase capillary leakage. The resulting loss of vascular tone and fluid from the circulation contributes to hypotension, allowing researchers to connect immune activation with circulatory failure.
Cytokines help indicate the intensity and character of the host inflammatory response, while endothelial cells help regulate the barrier between blood and tissues. In septic shock models, examining both processes links immune signaling to vascular dysfunction. This combined view is important for understanding how inflammation can progress from pathogen recognition to impaired circulation and tissue injury.
Models can expose cells, tissues, or whole organisms to microbial products or infectious agents and then examine interactions among pathogens, immune cells, and host tissues. Comparing these experimental systems helps researchers investigate whether observed changes reflect immune activation, pathogen-related effects, or their interaction. This distinction supports more focused study of disease mechanisms and therapeutic responses.
Key outcomes include cytokine release, endothelial dysfunction, vasodilation, capillary leakage, hypotension, and signs of multiple-organ injury. Together, these measurements show whether an experimental system captures linked inflammatory and circulatory changes rather than an isolated immune response. Researchers can also assess biomarkers to relate the model’s biological changes to disease severity and treatment effects.
Cellular, ex vivo, and animal systems provide complementary levels of investigation. Cellular models allow focused examination of interactions involving immune cells or host tissues, whereas ex vivo systems preserve tissue-level responses outside the body. Animal models enable assessment of broader circulatory changes and multiple-organ injury. Each system captures different aspects of septic shock-associated disease.
Selection depends on which part of septic shock the study aims to examine. A focused cellular system may suit interactions between pathogens and immune cells, while an ex vivo approach can address responses in host tissues. Animal models are relevant when investigators need broader circulatory or organ-level outcomes. Careful matching improves interpretation of mechanisms and treatment results.
Researchers monitor biomarkers alongside inflammatory, vascular, circulatory, and organ-related outcomes. These measurements can reveal whether a candidate intervention changes cytokine release, endothelial dysfunction, capillary leakage, hypotension, or multiple-organ injury. Comparing treated and untreated experimental systems helps determine which biological responses are modified and whether the model provides useful evidence about therapeutic strategies.
Septic shock reflects interactions among infectious agents, immune cells, and host tissues, so no single experimental system represents every aspect of the condition. Choosing a model that matches the scientific question helps investigators study relevant immune mechanisms, vascular consequences, or organ injury. This improves the reliability of conclusions about disease processes and potential treatments.