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Bioengineering
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重组胶原蛋白 i. 肽载体在生物反应器中的应用及其作为细胞传递系统的潜力
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
Please note that all translations are automatically generated.
Click here for the English version.
重组胶原蛋白 i. 肽载体在生物反应器中的应用及其作为细胞传递系统的潜力
DOI:
10.3791/57363-v
•
08:43 min
•
February 07, 2018
•
Melva Suarez Muñoz
,
Davide Confalonieri
,
Heike Walles
2
,
Elisabeth M. W. M. van Dongen
,
Gudrun Dandekar
2
1
Department Tissue Engineering and Regenerative Medicine
,
University Hospital Wuerzburg
,
2
Translational Center Regenerative Therapies (TLC-RT)
,
Fraunhofer Institute for Silicate Research ISC
,
3
Fujifilm Manufacturing Europe B.V.
Chapters
00:05
Title
00:55
Set-up and Sterilization of Spinner Flasks, Recombinant Collagen I Peptide (RCP) Microcarriers, and Bioreactor
02:09
Culture of hBMSCs and HDMECs on RCP Macroporous Microcarriers
03:29
Biomatrix Seeding and Start of Bioreactor System for HDMECs
05:05
Addition of RFP-HDMECs to the Biomatrix
06:16
Results: Cell Expansion on Macroporous Microcarriers and Cell Delivery to a Biomatrix in a Bioreactor Model
07:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
提出了大孔载体的细胞扩展协议及其在灌注生物反应器中的传递系统, 以细胞组织基质为种子。我们还包括不同的技术, 以确定细胞增殖和细胞在载体培养的生存能力。此外, 我们还展示了生物反应器培养后细胞的功能。
Tags
Recombinant Collagen I
Peptide Microcarriers
Cell Expansion
Cell Delivery
Bioreactor
Vascularization
Spinner Flask
Macroporous Microcarriers
Dulbecco’s Phosphate Buffered Saline
Autoclave
Culture Medium
Human Bone Marrow Derived Cells
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