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DOI: 10.3791/57429-v
This study presents a microfluidic device designed for multiple particle tracking microrheology measurements. It focuses on understanding the rheological effects of repeated phase transitions in soft matter.
We demonstrate the fabrication and use of a microfluidic device that enables multiple particle tracking microrheology measurements to study the rheological effects of repeated phase transitions on soft matter.
The overall goal of this procedure is to use microfluidics to induce consecutive phase transitions in a single soft matter sample by exchanging the surrounding fluid while simultaneously measuring changes in the rheological properties using multiple particle tracking microrheology. This method can answer key questions in the soft matter field, such as the impact of phase transitions, including molecular or colloidal rearrangement on dynamic rheological properties. This technique's main advantage is that the sample remains in place during multiple phase transitions, enabling real-time characterization.
This allows quantification of rheological properties, especially unique properties in heterogeneous systems. To begin the procedure, print the microfluidic device negative on a clear acetate sheet, and warm up a high-intensity UV lamp. On a second clear acetate sheet, trace the corners of a 75 millimeter by 50 millimeter slide.
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