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JoVE Journal
Neuroscience

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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
 

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Article DOI: 10.3791/57431
April 27th, 2018

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Summary April 27th, 2018

We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.

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