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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

DOI:
10.3791/57431-v

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09:20 min

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April 27, 2018

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, ,
1Centre for Discovery Brain Sciences,University of Edinburgh

Chapters

  • 00:04Title
  • 01:05Homogenization
  • 04:40Microglia Immunostaining
  • 06:41Results: Isolation of Neurons, Macrophages, and Microglia, and their RNA from Larval Zebrafish Brains
  • 08:25Conclusion

Summary

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We present a protocol to isolate neurons, macrophages and microglia from larval zebrafish brains under physiological and pathological conditions. Upon isolation, RNA is extracted from these cells to analyze their gene expression profile. This protocol allows for the collection of high-quality RNA for performing downstream analysis like qPCR and transcriptomics.

Tags

Keywords: IsolationRNA ExtractionNeuronsMacrophagesMicrogliaLarval Zebrafish BrainsTransgenic ZebrafishLive ImagingGene ExpressionCell SortingCentral Nervous SystemPTUFluorescent StereomicroscopeGFPDsREDTricaineHomogenizerMedia ARNA PurificationQPCRTranscriptomics

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