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JoVE Journal
Immunology and Infection

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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
 

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration

Article DOI: 10.3791/57435
May 7th, 2018

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Summary May 7th, 2018

Here we describe a protocol for establishing and culturing human- and mouse-derived 3-dimensional (3D) gastric organoids, and the method for the transfer of 3D organoids to a 2-dimensional monolayer. The use of the gastric epithelial monolayer as a novel scratch-wound assay for regeneration studies is also described.

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