Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Diego Marin; Alexandra L. Nguyen; Richard T. Scott, Jr.; Karen Schindler

doi:10.3791/57442

Journal

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Genetics

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Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

JoVE Journal
Genetics

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JoVE Journal Genetics

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

8,854 Views

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11:13 min

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April 10, 2018

DOI:

10.3791/57442-v

DOI:

10.3791/57442-v

•

11:13 min

April 10, 2018

•

8832 Views

Diego Marin*^1,2, Alexandra L. Nguyen*³, Richard T. Scott, Jr.^1,2, Karen Schindler³

¹IVI-RMA New Jersey ²Jefferson College of Biomedical Sciences,Thomas Jefferson University ³Department of Genetics,Rutgers, The State University of New Jersey

Summary

As the genetic variants associated with human disease begin to become uncovered, it is becoming increasingly important to develop systems with which to rapidly evaluate the biological significance of those identified variants. This protocol describes methods for evaluating human gene function during female meiosis I using mouse oocytes.