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足月胎盘胎膜中原发性人蜕细胞的分离
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Medicine
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JoVE Journal
Medicine
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
Please note that all translations are automatically generated.
Click here for the English version.
足月胎盘胎膜中原发性人蜕细胞的分离
DOI:
10.3791/57443-v
•
07:37 min
•
April 30, 2018
•
Tali Farine
2
,
Michael Parsons
,
Stephen Lye
2,3
,
Oksana Shynlova
2,3
1
Department of Physiology
,
University of Toronto
,
2
Lunenfeld-Tanenbaum Research Institute
,
Sinai Health System
,
3
Department of Obstetrics and Gynecology
,
University of Toronto
Chapters
00:05
Title
00:42
Collection of Decidual Tissue from Term Placental Membranes
01:57
Washing and Enzymatic Digestion of the Decidual Tissue
03:56
Generation of Single Cell Suspension
05:22
Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
07:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议展示了一种从足月胎盘的胎儿细胞膜中分离出的主要人类蜕细胞的方法, 可用于各种应用 (
即
免疫细胞化学、流式术中、
等
), 目的是研究不同细胞群在妊娠并发症中的作用。
Tags
Primary Decidual Cells
Human Reproduction
Blastocyst Implantation
Labor Initiation
Pregnancy-related Pathologies
Cell Isolation Protocol
Term Placenta
Enzymatic Digestion
Cell Viability
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