Journal
/
Neuroscience
/
Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

DOI:
10.3791/57444-v

•

07:25 min

•

March 27, 2018

•

, , , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Chapters

  • 00:04Title
  • 01:00Preparation of Fresh Frozen Brain Sections
  • 02:35RNA In Situ Hybridization Fluorescent Multiplex Assay
  • 05:12Results: Drd1α RNA Level in Nucleus Accumbens Region of F344/N Rats
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

Identification of dopamine D1-alpha receptor in the nucleus accumbens is critical for clarifying D1 receptor dysfunction during a central nervous system disease. We performed a novel RNA in situ hybridization assay to visualize single RNA molecules in a specific brain area.

Tags

Keywords: RNA In-situ HybridizationDopamine D1-alpha ReceptorNucleus AccumbensCentral Nervous System DiseaseDopaminergic DysfunctionTarget MRNABrain SectioningPre-treatmentMultiplex Assay

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57444?article_type=v

Simultaneous fMRI and Electrophysiology in the Rodent Brain
check_url/57444?article_type=v

VisioTracker, an Innovative Automated Approach to Oculomotor Analysis
check_url/57444?article_type=v

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
check_url/57444?article_type=v

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
check_url/57444?article_type=v

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
check_url/57444?article_type=v

Interfacing 3D Engineered Neuronal Cultures to Micro-Electrode Arrays: An Innovative In Vitro Experimental Model
check_url/57444?article_type=v

Simultaneous Detection of c-Fos Activation from Mesolimbic and Mesocortical Dopamine Reward Sites Following Naive Sugar and Fat Ingestion in Rats
check_url/57444?article_type=v

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression
check_url/57444?article_type=v

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
check_url/57444?article_type=v

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

Read Article