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Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons
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Neuroscience
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JoVE Journal Neuroscience
Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

DOI:
10.3791/57465-v

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05:25 min

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March 15, 2018

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, ,
1Department of Zoology and Physiology and Program in Neuroscience,University of Wyoming

Chapters

  • 00:05Title
  • 00:53Whole Brain Preparation
  • 03:15Horizontal Brain Slice Preparation
  • 04:05Results: Recordings from a Horizontal Brain Slice Preparation
  • 04:48Conclusion

Summary

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In this paper, we discuss three brain preparations used for whole cell patch clamp recording to study the retinotectal circuit of Xenopus laevis tadpoles. Each preparation, with its own specific advantages, contributes to the experimental tractability of the Xenopus tadpole as a model to study neural circuit function.

Tags

Keywords: Whole Cell Patch ClampXenopus LaevisTectal NeuronsNeural DevelopmentNeural CircuitsElectrophysiological RecordingOptic TectumAnesthetized TadpoleDissectionBrain PreparationStimulating ElectrodeOptic Chiasm

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