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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

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09:44 min

June 13, 2018

DOI:

09:44 min
June 13, 2018

8104 Views
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Summary

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Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

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