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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
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Chapters
- 00:04Title
- 00:53Preparation of Neutralized Collagen
- 01:55Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
- 04:08Image Acquisition Using Light-sheet Microscopy
- 05:07Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
- 07:17Results: Visualization of Human CTL Cells by Light-sheet Microscopy
- 08:32Conclusion
Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.
