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JoVE Journal
Immunology and Infection

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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
 

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Article doi: 10.3791/57651
June 13th, 2018

Summary June 13th, 2018

Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

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