Journal
/
Neuroscience
/
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

A Simple and Reproducible Method to Prepare Membrane Samples from Freshly Isolated Rat Brain Microvessels

DOI:
10.3791/57698-v

•

07:13 min

•

May 07, 2018

•

, , ,
1Department of Pharmacology, College of Medicine,University of Arizona, Tucson

Chapters

  • 00:04Title
  • 00:54Extraction and Processing of Brain Tissue from Sprague-Dawley Rats
  • 02:59Centrifugation
  • 04:27Results: Analysis of Freshly Isolated Rat Brain Microvessel Samples
  • 06:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a method for isolation of rat brain microvessels and for the preparation of membrane samples is described. This protocol has the clear advantage of producing enriched microvessel samples with acceptable protein yield from individual animals. Samples can then be used for robust protein analyses at the brain microvascular endothelium.

Tags

Keywords: Brain MicrovesselsBlood-brain BarrierRat BrainCerebral CortexProtease InhibitorBiochemical StudiesMembrane Isolation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57698?article_type=v

The Vermicelli and Capellini Handling Tests: Simple quantitative measures of dexterous forepaw function in rats and mice
check_url/57698?article_type=v

Gene Delivery to Postnatal Rat Brain by Non-ventricular Plasmid Injection and Electroporation
check_url/57698?article_type=v

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes
check_url/57698?article_type=v

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain
check_url/57698?article_type=v

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
check_url/57698?article_type=v

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila
check_url/57698?article_type=v

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
check_url/57698?article_type=v

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster
check_url/57698?article_type=v

Simple Generation of a High Yield Culture of Induced Neurons from Human Adult Skin Fibroblasts
check_url/57698?article_type=v

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Read Article