Journal
/
Neuroscience
/
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice

DOI:
10.3791/57781-v

•

07:51 min

•

June 28, 2018

•

, , ,
1Department of Physiology,Kanazawa Medical University

Chapters

  • 00:04Title
  • 00:44Construction of the Glass Optrode Holder
  • 01:29Head Post Installation
  • 04:04Craniotomy
  • 05:01Electrophysiological Recording
  • 06:33Results: The Light-evoked Responses in the IC of Awake VGAT-ChR2 Mice
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

This work introduces a method to perform an optogenetic single-unit recording reliably from an awake mouse using a custom-made glass optrode.

Tags

Keywords: OptogeneticsNeuroscienceNeuronal Type IdentificationGlass OptrodeIn VivoTransgenic MouseChannelrhodopsin-IIStereotaxic SurgeryDental CementHead Post

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57781?article_type=v

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
check_url/57781?article_type=v

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
check_url/57781?article_type=v

Preparation of an Awake Mouse for Recording Neural Responses and Injecting Tracers
check_url/57781?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/57781?article_type=v

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals
check_url/57781?article_type=v

Super-resolution Imaging of Neuronal Dense-core Vesicles
check_url/57781?article_type=v

Inducing Plasticity of Astrocytic Receptors by Manipulation of Neuronal Firing Rates
check_url/57781?article_type=v

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice
check_url/57781?article_type=v

Combining Optogenetics with Artificial microRNAs to Characterize the Effects of Gene Knockdown on Presynaptic Function within Intact Neuronal Circuits
check_url/57781?article_type=v

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Read Article