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JoVE Journal Bioengineering
Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
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Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Article DOI: 10.3791/57826-v • 16:20 min • July 2nd, 2018
July 2nd, 2018 •
*1, *2,3, 2, 4, 4, 3, 2,5
1Department of Mechanical Engineering, McGill University Montreal, 2Department of Bioengineering, McGill University Montreal, 3Department of Molecular Biology, Instituto Potosino de Investigación Científica y Tecnológica, A.C. (IPICyT), 4Department of Mining and Materials Engineering, McGill University Montreal, 5Department of Biomedical Engineering, McGill University Montreal
* These authors contributed equally

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Summary
Automatic Translation

We developed a heterogeneous breast cancer model consisting of immortalized tumor and fibroblast cells embedded in a bioprintable alginate/gelatin bioink. The model recapitulates the in vivo tumor microenvironment and facilitates the formation of multicellular tumor spheroids, yielding insight into the mechanisms driving tumorigenesis.

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Bioprintable Alginate Gelatin Hydrogel 3D In Vitro Model Systems Cell Spheroid Formation Cancer Biology Tumor Formation Cell Migration Tracking Cancer Signaling Healthy Cell Signaling 3D Environment Stromal Cells Fibroblasts Low Cost High Reproducibility Bioprintable Materials Biofunctional Materials Rheological Properties Cell Compatibility Hydrogel Preparation Cell Hydrogel Mixing Biosafety Cabinet Alginate Powder Gelatin Powder DPBS Solution Sterile Paraffin Film Aluminum Foil Polar Plots Electromyography (EMG) Phase Transition Liquid State Conical Centrifuge Tubes
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