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Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation
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1Department of Mechanical Engineering,McGill University Montreal, 2Department of Bioengineering,McGill University Montreal, 3Department of Molecular Biology,Instituto Potosino de Investigación Científica y Tecnológica, A.C. (IPICyT), 4Department of Mining and Materials Engineering,McGill University Montreal, 5Department of Biomedical Engineering,McGill University Montreal
Chapters
- 00:04Title
- 00:55Preparation of the A3G7 Hydrogel Precursor
- 02:49General Testing Protocol
- 04:15Measurements of Rheological Properties of Hydrogels
- 08:15Creating a Cell-laden A3 G7 Hydrogel Precursor
- 11:143D Printing Models
- 12:52Results: Cell Spheroid Formation Induced by 3D Bioprintable Hydrogel Systems
- 14:50Conclusion
We developed a heterogeneous breast cancer model consisting of immortalized tumor and fibroblast cells embedded in a bioprintable alginate/gelatin bioink. The model recapitulates the in vivo tumor microenvironment and facilitates the formation of multicellular tumor spheroids, yielding insight into the mechanisms driving tumorigenesis.
