Bioprintable Alginate/Gelatin Hydrogel 3D <em>In Vitro</em> Model Systems Induce Cell Spheroid Formation

Tao Jiang; Jose Munguia-Lopez; Salvador Flores-Torres; Joel Grant; Sanahan Vijayakumar; Antonio De Leon-Rodriguez; Joseph M. Kinsella

doi:10.3791/57826

Journal

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Bioengineering

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Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Bioprintable Alginate/Gelatin Hydrogel 3D <em>In Vitro</em> Model Systems Induce Cell Spheroid Formation

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

18,593 Views

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16:20 min

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July 02, 2018

DOI:

10.3791/57826-v

DOI:

10.3791/57826-v

•

16:20 min

July 02, 2018

•

18560 Views

Tao Jiang*¹, Jose Munguia-Lopez*^2,3, Salvador Flores-Torres², Joel Grant⁴, Sanahan Vijayakumar⁴, Antonio De Leon-Rodriguez³, Joseph M. Kinsella^2,5

¹Department of Mechanical Engineering,McGill University Montreal ²Department of Bioengineering,McGill University Montreal ³Department of Molecular Biology,Instituto Potosino de Investigación Científica y Tecnológica, A.C. (IPICyT)⁴Department of Mining and Materials Engineering,McGill University Montreal ⁵Department of Biomedical Engineering,McGill University Montreal

Summary

We developed a heterogeneous breast cancer model consisting of immortalized tumor and fibroblast cells embedded in a bioprintable alginate/gelatin bioink. The model recapitulates the in vivo tumor microenvironment and facilitates the formation of multicellular tumor spheroids, yielding insight into the mechanisms driving tumorigenesis.