Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Louise Benning; Samuel Robinson; Marie Follo; Lukas Andreas Heger; Daniela Stallmann; Daniel Duerschmied; Christoph Bode; Ingo Ahrens; Marcus Hortmann

doi:10.3791/57950

Journal

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Medicine

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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

JoVE Journal
Medicine

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JoVE Journal Medicine

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

8,108 Views

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04:41 min

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July 03, 2018

DOI:

10.3791/57950-v

DOI:

10.3791/57950-v

•

04:41 min

July 03, 2018

•

8066 Views

Louise Benning¹, Samuel Robinson^1,2, Marie Follo³, Lukas Andreas Heger¹, Daniela Stallmann¹, Daniel Duerschmied¹, Christoph Bode¹, Ingo Ahrens^1,4, Marcus Hortmann¹

¹Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg ²Department of Medicine,Monash University ³Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg ⁴Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne

Summary

Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.