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Digital PCR for kvantificering af cirkulerende MicroRNA i akut myokardieinfarkt og hjerte-kar-sygdom
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Digital PCR for kvantificering af cirkulerende MicroRNA i akut myokardieinfarkt og hjerte-kar-sygdom

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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Cirkulerende MicroRNA har vist lovende som biomarkører for hjerte-kar-sygdomme og akut myokardieinfarkt hjertet. I denne undersøgelse beskriver vi en protokol for miRNA udvinding, reverse transkription og digital PCR for den absolutte kvantificering af miRNAs i serum hos patienter med hjerte-kar-sygdom.

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Medicin sag 137 myokardieinfarkt hjerte-kar-sygdom digital PCR MicroRNA koronararteriesygdom serum biomarkører
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