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Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
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Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction

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9,935 Views

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08:31 min

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July 26, 2018

DOI:

10.3791/57953-v

DOI:
10.3791/57953-v

•

08:31 min
July 26, 2018

•

1 Views
, , , ,
1Department of Internal Medicine and Cardiology,Charité University Medicine, 2German Center for Cardiovascular Research (DZHK)

Summary

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Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.

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Atrial CardiomyocytesRat ModelMetabolic SyndromeHeart FailurePreserved Ejection FractionLangendorff ApparatusCannulationCardiomyocyte IsolationExcitation-contraction-coupling

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Cite this Article

Bode, D., Guthof, T., Pieske, B. M., Heinzel, F. R., Hohendanner, F. Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction. J. Vis. Exp. (137), e57953, doi:10.3791/57953 (2018).

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