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DOI: 10.3791/57969-v
Katherine M. Martin1, Elizabeth A. Hasenmueller2, John R. White3, Lisa G. Chambers4, Jeremy L. Conkle1
1Department of Physical and Environmental Sciences,Texas A&M University-Corpus Christi, 2Department of Earth and Atmospheric Sciences,Saint Louis University, 3Department of Oceanography and Coastal Sciences,Louisiana State University, 4Department of Biology,University of Central Florida
Most microplastic research to date has occurred in marine systems where suspended solid levels are relatively low. Focus is now shifting to freshwater systems, which may feature high sediment loads and floating debris. This protocol addresses collecting and analyzing microplastic samples from aquatic environments that contain high suspended solid loads.
This method will help researchers quantify microplastic loads in rivers, which often contain high sediment loads, as they are a major source of plastic debris to the ocean. The main advantage of this technique is that it enables filtering and sorting of microplastics and water samples with high sediment loads at sizes not typically included in previous studies. To begin rinse the filtration device and nylon mesh sieves three times with deionized water.
Then place the mesh sieves into each union joint, with the pour sizes decreasing from top to bottom. To prevent leaks seal each joint tightly. Next wet a mixed cellulose ester membrane filter with deionized water.
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