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Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
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Neuroscience
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JoVE Journal Neuroscience
Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology

DOI:
10.3791/58031-v

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08:17 min

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July 03, 2018

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, , ,
1School of Pharmacy,University of Wyoming, 2Department of Zoology and Physiology,University of Wyoming

Chapters

  • 00:04Title
  • 00:36Surgery and In Vivo Nerve Fixation
  • 02:32Osmium Tetroxide Treatment and Resin Embedding
  • 04:38Sectioning by Ultramicrotome
  • 05:58Toluidine Blue Staining
  • 06:47Results: Transverse Section Images of a Rat Sciatic Nerve Stained with Toluidine Blue
  • 07:48Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to visualize fine structures of peripheral nerves by obtaining and staining 1-2 µm sections with toluidine blue

Tags

Toluidine Blue StainingResin-embedded SectionsPeripheral Nerve MorphologyNerve InjuryNerve RegenerationSciatic NerveNerve FixationNerve SegmentationOsmium TetroxideEpoxy Embedding

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