Journal
/
Immunology and Infection
/
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

DOI:
10.3791/58074-v

•

07:22 min

•

August 14, 2018

•

, , , , ,
1Division of Infectious Diseases, Department of Medicine,Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology,New Jersey City University

Chapters

  • 00:04Title
  • 00:54Preparation of Target Cells for Cell-to-Cell Virus Transmission
  • 02:11Preparation of Donor Cells
  • 03:56Co-culture
  • 05:06Fluorescence-activated Cell Sorting
  • 05:35Results: Identification of a Novel HIV-1 Viral Membrane Fusion Inhibitor
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.

Tags

Keywords: Cre-LoxViral Membrane FusionHIV-1Drug DiscoveryHigh-throughput ScreeningHIV Fusion InhibitorsBiosafetyJurkat RG Reporter CellsPuromycinCell-to-cell Virus TransmissionGag-iCre DNAElectroporationFicoll

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58074?article_type=v

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
check_url/58074?article_type=v

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
check_url/58074?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/58074?article_type=v

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
check_url/58074?article_type=v

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
check_url/58074?article_type=v

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors
check_url/58074?article_type=v

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
check_url/58074?article_type=v

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
check_url/58074?article_type=v

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
check_url/58074?article_type=v

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

Read Article