Cre-lox Activated Assay
Cre-lox Activated Assay - Related Videos
08:13
A Cre-Lox P Recombination Approach for the Detection of Cell Fusion In Vivo
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Cited by 11 •
2012
A method to track cell fusion in living organisms over time is described. The approach utilizes Cre-LoxP recombination to induce luciferase expression upon cell fusion. The luminescent signal generated can be detected in living organisms using biophotonic imaging systems with a sensitivity of detection of ˜1,000 cells in peripheral tissues.
07:22
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
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Cited by 8 •
2018
We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.
10:29
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
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Cited by 6 •
2017
Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.
07:48
Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
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Cited by 3 •
2015
Intestinal epithelial stem cells (ISCs) are intermingled with Paneth cells. These cells are differentiated progeny of the ISC, which support the ISCs and provide antibacterial protection. Here we demonstrate how we used transgenic conditional mouse models to establish that Paneth cells play a crucial role in maintaining the intestinal epithelia.
08:19
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
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Cited by 15 •
2011
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
FAQs
Cre acts at paired loxP sites positioned around an intervening DNA segment. When the relevant process produces or delivers Cre, that segment is removed, allowing the previously silent reporter to become active. Because the DNA change remains after the initiating signal disappears, the assay preserves evidence that a cell experienced the defined event rather than showing only its immediate state.
The paired loxP arrangement creates the DNA target that Cre recognizes during reporter activation. Its placement around the intervening sequence determines whether recombinase activity can convert the reporter from silent to active. This design links the genetic readout to Cre exposure, helping identify cells or organisms in which the selected biological process occurred.
Cre production or delivery serves as the connection between the biological event and the reporter response. If infection or a pathogen-associated interaction supplies the Cre signal, reporter activation marks the affected cell or organism. Interpreting the result therefore depends on understanding which process generated Cre and which population received that signal.
Reporter activation records the DNA recombination event, so expression can remain detectable after Cre production or delivery has ended. The resulting signal can therefore identify cells that experienced a transient infection-related or pathogen-associated interaction, even when the initiating stimulus is no longer present. This makes the readout useful for reconstructing exposure history.
An experiment first links the event of interest to Cre production or delivery, then uses cells or organisms carrying the silent reporter configuration. After the biological process occurs, Cre-mediated removal of the intervening sequence activates the reporter in affected targets. Researchers can then analyze reporter-positive cells to examine infection dynamics, host responses, or cellular fate.
The assay can identify cells associated with infection, trace pathogen-associated interactions, and retain a record of transient exposure. Those marked populations can support analysis of how host responses develop, how infection changes over time, and what happens to affected cells afterward. Its stable genetic readout is especially useful when the initiating signal is brief or no longer detectable.