Name: Detection of Viruses from Bioaerosols Using Anion Exchange Resin (Video) | JoVE Journal
Uploaded: 2018-08-22T14:45:00.000+00:00
Duration: 6 min 10 s
Description: Learn an anion exchange resin method for sensitive virus detection from bioaerosols using liquid impingers. This technique improves molecular detection sensitivity and enables field-based public health research.

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5 minutes

This work was supported by funding from the CDC/NIOSH High Plains Intermountain Center for Agricultural Health and Safety (5U54OH008085) and the Colorado Bioscience Discovery Evaluation Grant Program (14BGF-16).

1. Setup of the Bioaerosol Chamber (See Figure 2)
<ol>
	<li>Pre-load the liquid impingers with 20 mL of 0.01 M phosphate buffered saline, pH 7.5 (PBS).
	<ol>
		<li>Add 0.5 g of anion exchange resin and suspend within the PBS of one of the liquid impingers, with another liquid impinger serving as a control.</li>
	</ol>
	</li>
	<li>Position liquid impingers in parallel inside the bioaerosol chamber using clamp stands with aerosol inlets facing the nebulizer. 
	NOTE: See Figure 2 for additional detail.</li>
	<li>Co-locate a direct-read aerosol monitor near the liquid impingers to measure mass concentration (mg/m3) of the bioaerosol.</li>
	<li>Co-locate additional direct-read instrumentation (indoor air quality monitor and thermal anemometer) near the liquid impingers to monitor environmental variables, such as temperature, relative humidity and wind speed, within the bioaerosol chamber.</li>
	<li>Run axial fans in the corners of the bioaerosol chamber to facilitate mixing of aerosol. 
	NOTE: Axial fans run at a fixed speed and are not adjustable.</li>
</ol>
2. Model Bioaerosolization Experiments (see Figure 3)
<ol>
	<li>Perform serial, 10-fold dilutions of MS2 bacteriophage and influenza vaccine to desired concentrations in phosphate-buffered saline (PBS). 
	NOTE: To demonstrate the method here, 103.5 FFU/mL of the type A and B influenza virus vaccine strains and 105 PFU/mL of the MS2 bacteriophage are utilized. To determine titers for the inocula, bacteriophages are propagated in Escherichia coli HS (pFamp)R and enumerated as previously described20. The commercial vaccine contains known quantities of four live attenuated influenza virus strains, two type A influenza viruses and two type B influenza viruses, expressed in fluorescent focus units or FFU.</li>
	<li>Prepare inocula within the glass vessel of a 6-jet collision nebulizer by creating suspensions of desired concentrations of viral particles in 100 mL of PBS and install within the custom bioaerosol chamber.</li>
	<li>Initiate instrumentation to measure variables within the bioaerosol chamber, such as temperature, % relative humidity, carbon dioxide concentration, and wind speed (generated using an axial fan). Use a direct-read aerosol monitor to track mass concentration within the bioaerosol chamber and to establish consistency within and between trials.</li>
	<li>Seal the bioaerosol chamber and purge for 10 min with HEPA-filtered air.</li>
	<li>Deliver filtered, dried air to the nebulizer operating at 6.9 kPa.</li>
	<li>Generate a target concentration of viral bioaerosols (via nebulizer) in each bioaerosol chamber trial. For this demonstration, bioaerosols with a concentration of 5 mg/m3 are generated.</li>
	<li>Once the target mass concentration of the aerosol has been reached, stop nebulization.</li>
	<li>Operate an air sampling pump for each liquid impinger that has been calibrated to a flow rate of 12.5 L/min. Conduct calibration using a primary flow standard before and after each sampling event to ensure consistency in sampling volume. Supply dilution air using a HEPA-filtered line located near the nebulizer and maintain constant pressure in the chamber.</li>
	<li>Actively sample the bioaerosol chamber atmosphere for a period of 40 min to achieve a sampling volume of 500 L per sample. 
	NOTE: Sampling for 40 min at 12.5 L/min with two pumps in parallel allows for sampling of 1,000 L of the bioaerosol. Thus, at the completion of the experiment, it is expected that the majority of the air present in the bioaerosol chamber has been sampled and released into the environment via a HEPA-filtered system. This is evidenced by the decrease in particle concentration. Post-experiment, surfaces are decontaminated with 10% household bleach and 70% ethanol. Indicators are used in this demonstration; however, if known pathogenic organisms are used in this system, additional biosafety measures may be implemented as needed.</li>
</ol>
3. Nucleic Acid Extraction and qRT-PCR Detection
<ol>
	<li>Isolate nucleic acids directly from anion exchange resin and, for comparative purposes, from the liquid used within the liquid impingers. In this example, a RNA isolation procedure is described, but a similar protocol could be used for DNA viruses.
	<ol>
		<li>Nucleic acid isolation from the anion exchange resin.
		<ol>
			<li>Transfer all of the anion exchange resin-containing liquid of the liquid impinger to a sterile 50 mL conical tube.</li>
			<li>Allow the resin to settle, and slowly decant the liquid sample from the anion exchange resin. Use a 1 mL pipette tip to remove all of the remaining liquid from the anion exchange resin.</li>
			<li>From a viral RNA isolation kit, add 560 &#181;L of viral lysis buffer containing carrier RNA directly to the anion exchange resin and incubate for 10 min at room temperature with periodic mixing.</li>
			<li>Using a 1 mL pipette tip, transfer the viral lysate (the viral lysis buffer) to a sterile 1.5 mL conical tube and proceed with RNA isolation using a viral RNA isolation kit (see Table of Materials) in accordance with the manufacturer&#39;s instructions, except that RNA is eluted in a total volume of 60 &#181;L of elution buffer.</li>
		</ol>
		</li>
		<li>Nucleic acid isolation from liquid impingers.
		<ol>
			<li>Pipette 140 &#181;L of the liquid sample into a sterile 1.5 mL conical tube and perform RNA isolation using the viral RNA isolation kit in accordance with the manufacturer&#39;s instructions, with the exception of eluting RNA in a total volume of 60 &#181;L of elution buffer. 
			NOTE: 140 &#181;L is the maximum sample volume that can be processed with the specific viral RNA isolation kit employed here in a single-step preparation.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Perform qRT-PCR for detection of MS2 and influenza A and B viruses as previously described23,24. In this demonstration, 5 &#181;L of nucleic acid eluates are used for qRT-PCR.</li>
</ol>

<ol><li>Pirtle, E. C., Beran, G. W. <a target="_blank" href="https://www.ncbi.nlm.nih.gov/pubmed/1782426">Virus survival in the environment.</a> Revue scientifique et technique (International Office of Epizootics). 10 (3), 733-748 (1991).</li>
<li>Nguyen, T. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+community-wide+outbreak+of+legionnaires+disease+linked+to+industrial+cooling+towers--how+far+can+contaminated+aerosols+spread%3F%5BTitle%5D)+AND+%22The+Journal+of+Infectious+Diseases%22%5BJournal%5D)">A community-wide outbreak of legionnaires disease linked to industrial cooling towers--how far can contaminated aerosols spread?</a> The Journal of Infectious Diseases. 193 (1), 102-111 (2006).</li>
<li>Marks, P. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evidence+for+airborne+transmission+of+Norwalk-like+virus+%28NLV%29+in+a+hotel+restaurant%5BTitle%5D)+AND+%22Epidemiology+and+Infection%22%5BJournal%5D)">Evidence for airborne transmission of Norwalk-like virus (NLV) in a hotel restaurant.</a> Epidemiology and Infection. 124 (3), 481-487 (2000).</li>
<li>Marks, P. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+school+outbreak+of+Norwalk-like+virus%3A+Evidence+for+airborne+transmission%5BTitle%5D)+AND+%22Epidemiology+and+Infection%22%5BJournal%5D)">A school outbreak of Norwalk-like virus: Evidence for airborne transmission.</a> Epidemiology and Infection. 131 (1), 727-736 (2003).</li>
<li>Corzo, C. A., Culhane, M., Dee, S., Morrison, R. B., Torremorell, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Airborne+detection+and+quantification+of+swine+influenza+a+virus+in+air+samples+collected+inside%2C+outside+and+downwind+from+swine+barns%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Airborne detection and quantification of swine influenza a virus in air samples collected inside, outside and downwind from swine barns.</a> PLoS One. 8 (8), e71444(2013).</li>
<li>Anderson, B. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bioaerosol+sampling+in+modern+agriculture%3A+A+novel+approach+for+emerging+pathogen+surveillance%5BTitle%5D)+AND+%22The+Journal+of+Infectious+Diseases%22%5BJournal%5D)">Bioaerosol sampling in modern agriculture: A novel approach for emerging pathogen surveillance.</a> The Journal of Infectious Diseases. 214 (4), 537-545 (2016).</li>
<li>Hietala, S. K., Hullinger, P. J., Crossley, B. M., Kinde, H., Ardans, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Environmental+air+sampling+to+detect+exotic+Newcastle+disease+virus+in+two+California+commercial+poultry+flocks%5BTitle%5D)+AND+%22Journal+of+Veterinary+Diagnostic+Investigation%22%5BJournal%5D)">Environmental air sampling to detect exotic Newcastle disease virus in two California commercial poultry flocks.</a> Journal of Veterinary Diagnostic Investigation. 17 (2), 198-200 (2005).</li>
<li>Jonges, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Wind-mediated+spread+of+low-pathogenic+avian+influenza+virus+into+the+environment+during+outbreaks+at+commercial+poultry+farms%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Wind-mediated spread of low-pathogenic avian influenza virus into the environment during outbreaks at commercial poultry farms.</a> PLoS One. 10 (5), e0125401(2015).</li>
<li>Otake, S., Dee, S. A., Jacobson, L., Torremorell, M., Pijoan, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+aerosol+transmission+of+porcine+reproductive+and+respiratory+syndrome+virus+under+controlled+field+conditions%5BTitle%5D)+AND+%22The+Veterinary+Record%22%5BJournal%5D)">Evaluation of aerosol transmission of porcine reproductive and respiratory syndrome virus under controlled field conditions.</a> The Veterinary Record. 150 (26), 804-808 (2002).</li>
<li>Wu, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aerosolized+avian+influenza+A+%28H5N6%29+virus+isolated+from+a+live+poultry+market%2C+China%5BTitle%5D)+AND+%22The+Journal+of+Infection%22%5BJournal%5D)">Aerosolized avian influenza A (H5N6) virus isolated from a live poultry market, China.</a> The Journal of Infection. 74 (1), 89-91 (2017).</li>
<li>Choi, M. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Live+animal+markets+in+Minnesota%3A+A+potential+source+for+emergence+of+novel+influenza+A+viruses+and+interspecies+transmission%5BTitle%5D)+AND+%22Clinical+Infectious+Diseases%22%5BJournal%5D)">Live animal markets in Minnesota: A potential source for emergence of novel influenza A viruses and interspecies transmission.</a> Clinical Infectious Diseases. 61 (9), 1355-1362 (2015).</li>
<li>Haig, C. W., Mackay, W. G., Walker, J. T., Williams, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bioaerosol+sampling%3A+Sampling+mechanisms%2C+bioefficiency+and+field+studies%5BTitle%5D)+AND+%22The+Journal+of+Hospical+Infection%22%5BJournal%5D)">Bioaerosol sampling: Sampling mechanisms, bioefficiency and field studies.</a> The Journal of Hospical Infection. 93 (3), 242-255 (2016).</li>
<li>Anderson, B. D., Lednicky, J. A., Torremorell, M., Gray, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+use+of+bioaerosol+aampling+for+airborne+virus+surveillance+in+swine+production+facilities%3A+A+mini+review%5BTitle%5D)+AND+%22Frontiers+in+Veterinary+Science%22%5BJournal%5D)">The use of bioaerosol aampling for airborne virus surveillance in swine production facilities: A mini review.</a> Frontiers in Veterinary Science. 4, 121(2017).</li>
<li>Verreault, D., Moineau, S., Duchaine, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Methods+for+sampling+of+airborne+viruses%5BTitle%5D)+AND+%22Microbiology+and+Molecular+Biology+Reviews%22%5BJournal%5D)">Methods for sampling of airborne viruses.</a> Microbiology and Molecular Biology Reviews. 72 (3), 413-444 (2008).</li>
<li>Hogan, C. J. Jr <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sampling+methodologies+and+dosage+assessment+techniques+for+submicrometre+and+ultrafine+virus+aerosol+particles%5BTitle%5D)+AND+%22Journal+of+Applied+Microbiology%22%5BJournal%5D)">Sampling methodologies and dosage assessment techniques for submicrometre and ultrafine virus aerosol particles.</a> Journal of Applied Microbiology. 99 (6), 1422-1434 (2005).</li>
<li>Yu, K. -P., Chen, Y. -P., Gong, J. -Y., Chen, Y. -C., Cheng, C. -C. <a target="_blank" href="https://www.sciencedirect.com/science/article/pii/S0021850216300143">Improving the collection efficiency of the liquid impinger for ultrafine particles and viral aerosols by applying granular bed filtration.</a> Journal of Aerosol Science. 101, 133-143 (2016).</li>
<li>Perez-Mendez, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+a+simple+and+cost+effective+filter+paper-based+shipping+and+storage+medium+for+environmental+sampling+of+F-RNA+coliphages%5BTitle%5D)+AND+%22J+Virol+Methods%22%5BJournal%5D)">Evaluation of a simple and cost effective filter paper-based shipping and storage medium for environmental sampling of F-RNA coliphages.</a> J Virol Methods. 194 (1-2), 60-66 (2013).</li>
<li>Chandler, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Field-based+evaluation+of+a+male-specific+%28F%2B%29+RNA+coliphage+concentration+method%5BTitle%5D)+AND+%22Journal+of+Virological+Methods%22%5BJournal%5D)">Field-based evaluation of a male-specific (F+) RNA coliphage concentration method.</a> Journal of Virological Methods. 239, 9-16 (2017).</li>
<li>Perez-Mendez, A., Chandler, J. C., Bisha, B., Goodridge, L. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Concentration+of+enteric+viruses+from+tap+water+using+an+anion+exchange+resin-based+method%5BTitle%5D)+AND+%22Journal+of+Virological+Methods%22%5BJournal%5D)">Concentration of enteric viruses from tap water using an anion exchange resin-based method.</a> Journal of Virological Methods. 206, 95-98 (2014).</li>
<li>Perez-Mendez, A., Chandler, J. C., Bisha, B., Goodridge, L. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+an+anion+exchange+resin-based+method+for+concentration+of+F-RNA+coliphages+%28enteric+virus+indicators%29+from+water+samples%5BTitle%5D)+AND+%22Journal+of+Virological+Methods%22%5BJournal%5D)">Evaluation of an anion exchange resin-based method for concentration of F-RNA coliphages (enteric virus indicators) from water samples.</a> Journal of Virological Methods. 204, 109-115 (2014).</li>
<li>Kammerer, J., Carle, R., Kammerer, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Adsorption+and+ion+exchange%3A+Basic+principles+and+their+application+in+food+processing%5BTitle%5D)+AND+%22Journal+of+Agricultural+and+Food+Chemistry%22%5BJournal%5D)">Adsorption and ion exchange: Basic principles and their application in food processing.</a> Journal of Agricultural and Food Chemistry. 59 (1), 22-42 (2011).</li>
<li>Chandler, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+method+for+the+improved+detection+of+aerosolized+influenza+viruses+and+the+male-specific+%28F%2B%29+RNA+coliphage+MS2%5BTitle%5D)+AND+%22Journal+of+Virological+Methods%22%5BJournal%5D)">A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.</a> Journal of Virological Methods. 246, 38-41 (2017).</li>
<li>Friedman, S. D., Cooper, E. M., Calci, K. R., Genthner, F. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Design+and+assessment+of+a+real+time+reverse+transcription-PCR+method+to+genotype+single-stranded+RNA+male-specific+coliphages+%28Family+Leviviridae%29%5BTitle%5D)+AND+%22Journal+of+Virological+Methods%22%5BJournal%5D)">Design and assessment of a real time reverse transcription-PCR method to genotype single-stranded RNA male-specific coliphages (Family Leviviridae).</a> Journal of Virological Methods. 173 (2), 196-202 (2011).</li>
<li>Selvaraju, S. B., Selvarangan, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+three+influenza+A+and+B+real-time+reverse+transcription-PCR+assays+and+a+new+2009+H1N1+assay+for+detection+of+influenza+viruses%5BTitle%5D)+AND+%22Journal+of+Clinical+Microbiology%22%5BJournal%5D)">Evaluation of three influenza A and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses.</a> Journal of Clinical Microbiology. 48 (11), 3870-3875 (2010).</li>
<li>Cademartiri, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Immobilization+of+bacteriophages+on+modified+silica+particles%5BTitle%5D)+AND+%22Biomaterials%22%5BJournal%5D)">Immobilization of bacteriophages on modified silica particles.</a> Biomaterials. 31 (7), 1904-1910 (2010).</li>
<li>Michen, B., Graule, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Isoelectric+points+of+viruses%5BTitle%5D)+AND+%22Journal+of+Appled+Microbiology%22%5BJournal%5D)">Isoelectric points of viruses.</a> Journal of Appled Microbiology. 109 (2), 388-397 (2010).</li>
<li>Turgeon, N., Toulouse, M. J., Martel, B., Moineau, S., Duchaine, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparison+of+five+bacteriophages+as+models+for+viral+aerosol+studies%5BTitle%5D)+AND+%22Applied+and+Environmental+Microbiology%22%5BJournal%5D)">Comparison of five bacteriophages as models for viral aerosol studies.</a> Applied and Environmental Microbiology. 80 (14), 4242-4250 (2014).</li>
<li>Vergara, G. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evaluation+of+FRNA+coliphages+as+indicators+of+human+enteric+viruses+in+a+tropical+urban+freshwater+catchment%5BTitle%5D)+AND+%22Water+Research%22%5BJournal%5D)">Evaluation of FRNA coliphages as indicators of human enteric viruses in a tropical urban freshwater catchment.</a> Water Research. 79, 39-47 (2015).</li>
<li>Tung-Thompson, G., Libera, D. A., Koch, K. L., de Los Reyes, F. L. 3rd, Jaykus, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Aerosolization+of+a+human+norovirus+surrogate%2C+bacteriophage+MS2%2C+during+simulated+vomiting%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Aerosolization of a human norovirus surrogate, bacteriophage MS2, during simulated vomiting.</a> PLoS One. 10 (8), e0134277(2015).</li>
</ol>

The authors have nothing to disclose.

This protocol outlines a method for sensitive viral capture from bioaerosols using modified liquid impingers. The method is optimized for detection and quantification of the viral load in bioaerosols. The specific modification demonstrated here involves the addition of anion exchange resin to liquid contained within a common liquid impinger. This method was developed for its simplicity in downstream sample processing, whereas other sample processing techniques such as centrifugation, filtration, and precipitation-based m...

The purpose of this method is to provide a highly sensitive bioaerosol sampling platform to facilitate molecular detection of negatively-charged viruses from bioaerosols. Microorganisms, including viral particles, can survive in bioaerosols for extended periods of time1. Bioaerosols can travel over relatively long distances and maintain viability and infectivity, as evidenced by an outbreak of Legionnaires&#39; disease that originated from industrial cooling towers located at a distance of 6 km from the affected individuals and resulted in 18 fatalities2. Indirect transmission of viruses to humans mediated by bioaerosols can occur in multiple settings and has been demonstrated for norovirus outbreaks in schools and restaurants3,4. Similarly, bioaerosol transmission of viruses can occur in agricultural settings such as in swine and poultry farms, with this transmission route being considered as a major factor in the movement of viruses between production facilities5,6,7,8,9.
Effective sampling of virus-laden bioaerosols allows for improvement in rapid diagnostics and preparedness for outbreak prevention, as shown in demonstrations in which H5 influenza A viruses were detected from bioaerosols in live animal markets in China and the United States10,11. Current bioaerosol sampling technologies involve a number of different particle capture principles, and can be broadly categorized into impingers, cyclones, impactors, and filters12. It is beyond the scope of this protocol to exhaustively cover all advantages and disadvantages of these platforms for sampling of viruses from bioaerosols; however, it can be stated that the majority of these sampling devices have not been optimized for the collection of viruses and bacteriophages13. Furthermore, infectivity of viral particles is often negatively affected, with liquid impingers considered to maintain viral infectivity more effectively than sampling devices such as solid impactors or filters14. However, one disadvantage of liquid impingement is the target dilution effect, which occurs because viruses are collected in relatively large volumes (typically &#8805;20 mL) of liquid in the collection vessel. Another important disadvantage involves the suboptimal efficiency of liquid impingers to concentrate particles &#60;0.5 &#181;M in size15. However, capture efficiency of these devices can be improved by immobilization on solid matrices, as immobilization can enhance preservation of viral nucleic acids and viral infectivity16,17.
We have previously demonstrated that anion exchange resin is an effective tool for the capture and concentration of viruses from liquid matrices, including F-RNA bacteriophages, hepatitis A virus, human adenovirus, and rotavirus18,19,20. As defined by the manufacturer, the anion exchange resin utilized in this work is a macroreticular polystyrene strong base anion exchange resin in which functionalized quaternary amine groups mediate attraction and capture of anions in a liquid medium21. Consequently, the anion exchange resin is expected to capture viruses with net-negative surface charges, including many enteric viruses, influenza viruses and other viruses relevant to public and animal health.
The current protocol involves the addition of anion exchange resin to a liquid impinger. In this system, the resin serves as a secondary concentration step for viral particles captured in the impinger liquid. Nucleic acids can then be directly eluted in small volumes, providing a concentrated sample for molecular analyses. Thus, the main advantage of this method is the improvement in viral detection sensitivity, primarily through reduction in sample volume. Additionally, due to the inherent non-specific capture of negatively-charged viruses, the method is likely applicable for detection of a large number of viruses of interest. Here, the method is demonstrated for vaccine strains of type A and type B influenza viruses and the FRNA coliphage MS2 (MS2). These viruses are subsequently detected using standard qRT-PCR assays as previously described22. The end-point user should not expect to encounter difficulties in performing this method, because modifications to currently existing equipment do not constitute major disruptions to the conventional flow of bioaerosol sampling and analysis.

<table><tbody><tr><td>&lt;em&gt;Escherichia coli&lt;/em&gt; bacteriophage MS2 (ATCC 15597-B1)</td><td>American Type Culture Collection</td><td>ATCC 15597-B1</td><td></td></tr><tr><td>FluMist Quadrivalent</td><td>AstraZeneca</td><td>Contact manufacturer</td><td>Viral constitutents of this vaccine are subject to change on an annual basis</td></tr><tr><td>CFX96 Touch Real-Time PCR Detection System</td><td>Bio-Rad</td><td>1855195</td><td></td></tr><tr><td>Primers and probes</td><td>Integrated DNA Technologies</td><td>NA</td><td></td></tr><tr><td>0.2 &amp;micro;M sterile filter</td><td>NA</td><td>NA</td><td></td></tr><tr><td>1 L pyrex bottles or equivalent</td><td>NA</td><td>NA</td><td></td></tr><tr><td>1 mL pipet tips</td><td>NA</td><td>NA</td><td></td></tr><tr><td>1 mL pipettor</td><td>NA</td><td>NA</td><td></td></tr><tr><td>50 mL serological pipet</td><td>NA</td><td>NA</td><td></td></tr><tr><td>PCR tubes</td><td>NA</td><td>NA</td><td></td></tr><tr><td>Pipet-aid or equivalent</td><td>NA</td><td>NA</td><td></td></tr><tr><td>QIAamp Viral RNA Mini Kit</td><td>Qiagen</td><td>52904</td><td></td></tr><tr><td>QuantiTect Probe RT-PCR Kit</td><td>Qiagen</td><td>204443</td><td></td></tr><tr><td>Amberlite IRA-900 chloride form</td><td>Sigma-Aldrich</td><td>216585-500G</td><td></td></tr><tr><td>Phosphate buffered saline</td><td>Sigma-Aldrich</td><td>P5368-10PAK</td><td></td></tr><tr><td>Water (molecular biology grade)</td><td>Sigma-Aldrich</td><td>W4502-1L</td><td></td></tr><tr><td>Eppendorf DNA LoBind Microcentrifuge Tubes</td><td>ThermoFisher</td><td>13-698-791</td><td></td></tr><tr><td>Falcon 50 mL Conical Centrifuge Tubes&amp;nbsp;</td><td>ThermoFisher</td><td>14-432-22</td><td></td></tr><tr><td>Falcon Polypropylene Centrifuge Tubes</td><td>ThermoFisher</td><td>05-538-62</td><td></td></tr><tr><td>SuperScript III Platinum One-Step qRT-PCR Kit w/ROX</td><td>ThermoFisher</td><td>11745100</td><td></td></tr><tr><td>SKC Biosampler 20 mL, 3-piece glass set</td><td>SKC Inc.</td><td>225-9593</td><td></td></tr><tr><td>Vac-u-Go sample pumps</td><td>SKC Inc.</td><td>228-9695</td><td></td></tr><tr><td>Collison nebulizer (6-jet)</td><td>BGI Inc.</td><td>NA</td><td></td></tr><tr><td>HEPA capsule</td><td>PALL</td><td>12144</td><td></td></tr><tr><td>Q-TRAK indoor air quality monitor 8554</td><td>TSI Inc.</td><td>NA</td><td></td></tr><tr><td>Alnor velometer thermal anemometer AVM440-A</td><td>TSI Inc.</td><td>NA</td><td></td></tr><tr><td>SidePak AM510 personal aerosol monitor</td><td>TSI Inc.</td><td>NA</td><td></td></tr><tr><td>Bioaerosol chamber</td><td>NA</td><td>NA</td><td></td></tr></tbody></table>

detection of viruses from bioaerosols using anion exchange resin

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

This protocol demonstrates a customized bioaerosol sampling method for viruses. In this system, anion exchange resin is coupled with liquid impingement-based air sampling devices for efficacious concentration of negatively-charged viruses from bioaerosols. Thus, the resin serves as an additional concentration step in the bioaerosol sampling workflow. Nucleic acid extraction of the viral particles is then performed directly from the anion exchange resin, with the resulting sample suitable for molecular analyses. Further, this protocol describes a custom-built bioaerosol chamber capable of generating virus-laden bioaerosols under a variety of environmental conditions and allowing for continuous monitoring of environmental variables such as temperature, humidity, wind speed, and aerosol mass concentration. The main advantage of using this protocol is increased sensitivity of viral detection, as assessed via direct comparison to an unmodified conventional liquid impinger. Other advantages include the potential to concentrate diverse negatively-charged viruses, the low cost of anion exchange resin (~$0.14 per sample), and ease of use. Disadvantages include the inability of this protocol to assess infectivity of resin-adsorbed viral particles, and potentially the need for the optimization of the liquid sampling buffer used within the impinger.

Figure 1 demonstrates the principle behind charge-based capture of viruses from bioaerosols via inclusion of resin in liquid-based impingers. Figure 2 shows the setup of the custom-built bioaerosol chamber. Figure 3 describes the steps involved in setting up the aerosolization experiment and measures to ensure quality control. Figure 4 shows amplification curves for qRT-...

An anion exchange resin-based method, adapted to liquid impingement-based bioaerosol sampling of viruses is demonstrated. When coupled with downstream molecular detection, the method allows for facile and sensitive detection of viruses from bioaerosols.

This protocol demonstrates a customized bioaerosol sampling method for viruses. In this system, anion exchange resin is coupled with liquid ...

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Source: Schaeffer, J. W., Chandler, et al. Detection of Viruses from Bioaerosols Using Anion Exchange Resin. J. Vis. Exp. (2018).This video demonstrates the detection of viruses from bioaerosols using an anion exchange resin. A liquid impinger containing the resin is used to capture viruses from the air. The resin is collected and treated with a virus lysis buffer containing carrier RNA. The lysate is transferred to a microcentrifuge tube for viral RNA isolation, followed by qRT-PCR to amplify...

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Learn an anion exchange resin method for sensitive virus detection from bioaerosols using liquid impingers. This technique improves molecular detection sensitivity and enables field-based public health research.

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

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