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Developmental Biology
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低血清条件下心脏祖细胞内皮分化的诱导作用
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions
Please note that all translations are automatically generated.
Click here for the English version.
低血清条件下心脏祖细胞内皮分化的诱导作用
DOI:
10.3791/58370-v
•
12:48 min
•
January 07, 2019
•
Michika Mochizuki*
1
,
Giacomo Della Verde*
1
,
Habiba Soliman
,
Otmar Pfister
2
,
Gabriela M. Kuster
2
1
Department of Biomedicine
,
University Hospital and University of Basel
,
2
Division of Cardiology
,
University Hospital Basel
Chapters
00:04
Title
01:01
Tissue Preparation and Digestion
02:48
Filtration
04:43
Primary Culture of Isolated SP-CPCs
05:28
SP-CPC Growth and Viability Under Different Serum Concentrations
07:28
Induction of Endothelial Differentiation
08:59
Tube Formation Assay
10:15
Results
11:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方案描述了一种用于心脏祖细胞的内皮分化技术。它特别关注血清浓度和细胞播种密度如何影响内皮分化潜力。
Tags
Cardiac Progenitor Cells
Endothelial Differentiation
Low Serum Conditions
Cell Therapy
Cardiac Disease
Anesthetize
Collagenase B
Hank’s Balanced Salt Solution
Red Blood Cell Lysis Buffer
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