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1Department of Pharmacology and Toxicology,The University of Mississippi Medical Center, 2Department of Medicine, Division of Nephrology,Rush University Medical Center, 3Department of Medicine, Division of Nephrology,The University of Mississippi Medical Center, 4Department of Neurology,The University of Mississippi Medical Center
Chapters
- 00:04Title
- 01:34Kidney Isolation
- 02:33Kidney Homogenization, Incubation, and Straining
- 05:10Glomeruli Enumeration and Nephron Number Extrapolation
- 06:20Results: Representative Nephron Quantification
- 07:11Conclusion
Estimates of whole kidney nephron number are important clinically and experimentally, as there is an inverse association between nephron number and an enhanced risk of renal and cardiovascular disease.Herein, the use of the acid maceration method, which provides fast and reliable estimates of whole kidney nephron number, is demonstrated.
