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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

Article DOI: 10.3791/58648-v • 09:43 min • November 30th, 2018
November 30th, 2018 •
1,2, 1,2, 1,2
1Department of Psychiatry & Behavioral Sciences, University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation, University of Miami Miller School of Medicine

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Summary
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The purpose of this article is to provide a comprehensive, systematic guide to the efficient purification of histones H3 and H4 and the quantification of acetylated histone residues.

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Purification H3 H4 Histone Proteins Quantification Acetylated Histone Marks Cells Brain Tissue Method Core Histones Post-translational Modifications High Quality Reproducible Results Advantages Native Post-translational Modifications Low-throughput Methods Medium-throughput Applications Karolina Janczura Graduate Student Optimized Protocol Epigenetic Regulation Alzheimer's Disease Plate Cells Tissue Culture-treated Dishes Cell Culture Media Confluency Aspirate Culture Media Wash Cells Serum-free Media Extraction Buffer
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