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JoVE Journal Biochemistry
Fluoreszierende silberne Färbung von Proteinen im Polyacrylamid-Gele
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Fluoreszierende silberne Färbung von Proteinen im Polyacrylamid-Gele

Article DOI: 10.3791/58669-v • 06:24 min • April 21st, 2019
April 21st, 2019 •
1, 1, 2, 1
1Ming Wai Lau Centre for Reparative Medicine, Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science, Hong Kong University of Science and Technology

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Hier beschreiben wir ein detailliertes Protokoll umreißt eine neue fluoreszierende Färbung Technik für Gesamt-Protein Nachweis in Polyacrylamid-Gele. Das Protokoll nutzt eine Sonde, Silber Ionen-spezifische Fluoreszenz Turn-on, die Ag+erkennt-Proteinkomplexe, und gewisse Einschränkungen der traditionellen chromogenen Silber Flecken beseitigt.

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Biochemie Ausgabe 146 silberne Färbung fluoreszierende Gel Färbung Sodium Dodecyl Sulfat-Polyacrylamid-Gelelektrophorese (SDS-PAGE) Aggregation-induzierte Emission (AIE) Silber-Ionen-Sensor Fluorogenic Erkennung Proteomik
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