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A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

DOI:
10.3791/58672-v

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10:44 min

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August 09, 2019

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, , , , , , , , ,
1Surgery Branch,National Cancer Institute, NIH, 2Center for Cell-Based Therapy,National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research,National Cancer Institute, NIH

Chapters

  • 00:04Title
  • 00:57Preparation of OP9/DLL1 Cells for Co-culture with iPSC
  • 02:08In Vitro Differentiation of iPSC into Immature T Cells
  • 05:003D Thymic Organ Culture to Generate iTE
  • 08:00Results: Generation of Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants
  • 09:46Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a novel method to generate tumor antigen-specific induced pluripotent stem cell-derived thymic emigrants (iTE) by a three-dimensional (3D) thymic culture system. iTE are a homogenous subset of T cells closely related to naïve T cells with the capacity for proliferation, memory formation, and tumor suppression.

Tags

Keywords: 3D Thymic CultureInduced Pluripotent Stem CellsTumor Antigen-specific T CellsThymic EmigrantsOP9/DLL1 CellsTrypsinizationCell SuspensionCell CultureT Cell DifferentiationIn Vivo T Cell Generation

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