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Mikrofluid analyse til vurdering af leukocyt adhæsion til menneskelige induceret pluripotente stamceller-afledt endotelceller (hiPSC-ECs)
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
DOI:

06:47 min

November 26, 2018

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Chapters

  • 00:04Title
  • 00:36Microfluidic Chip Coating and Human Induced Pluripotent Stem Cell-derived Endothelial Cell (hiPSC-EC) Seeding
  • 02:17Live Imaging and Adherent Leukocyte Quantification
  • 05:21Results: Representative Adherent Leukocyte Identification
  • 06:09Conclusion

Summary

Automatic Translation

Denne trinvise protokol indeholder en detaljeret beskrivelse af eksperimentel opsætning og dataanalyse til vurdering af inflammatoriske respons i hiPSC-ECs og analyse af leukocyt adhæsion under fysiologiske flow.

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