Journal
/
Developmental Biology
/
Mikrofluid analyse til vurdering af leukocyt adhæsion til menneskelige induceret pluripotente stamceller-afledt endotelceller (hiPSC-ECs)
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Mikrofluid analyse til vurdering af leukocyt adhæsion til menneskelige induceret pluripotente stamceller-afledt endotelceller (hiPSC-ECs)

DOI:
10.3791/58678-v

•

06:47 min

•

November 26, 2018

•

, , ,
1Department of Anatomy and Embryology,Leiden University Medical Center, 2Department of Applied Stem Cell Technologies,University of Twente

Chapters

  • 00:04Title
  • 00:36Microfluidic Chip Coating and Human Induced Pluripotent Stem Cell-derived Endothelial Cell (hiPSC-EC) Seeding
  • 02:17Live Imaging and Adherent Leukocyte Quantification
  • 05:21Results: Representative Adherent Leukocyte Identification
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

Denne trinvise protokol indeholder en detaljeret beskrivelse af eksperimentel opsætning og dataanalyse til vurdering af inflammatoriske respons i hiPSC-ECs og analyse af leukocyt adhæsion under fysiologiske flow.

Tags

MicrofluidicLeukocyte AdhesionHuman Induced Pluripotent Stem Cell-derived Endothelial CellsBiochip CoatingCell SeedingLive ImagingMicrofluidic Pump

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Feeder-fri Udledning af melanocytter fra menneskelige pluripotente stamceller

Generation af induceret pluripotente stamceller fra human melanoma Tumor-infiltrerende lymfocytter

Pluripotente Stem Cell Afledt hjerteceller til Myokardie Repair

Hurtig, instrueret differentiering af retinale Pigment epitel celler fra menneskelige embryonale eller induceret pluripotente stamceller

Ved hjælp af menneskelige induceret pluripotente stamceller-afledt hepatocyt-lignende celler for Drug Discovery

En familiær hyperkolesterolæmi menneskelige lever kimære musen Model ved hjælp af induceret pluripotente stamceller-afledt hepatocytter

RNA-baserede omprogrammering af menneskelige primære fibroblaster til induceret pluripotente stamceller

Effektiv differentiering af humane pluripotente stamceller i leverceller

Generation af 3D hud Organoid fra ledningen blod-afledte induceret pluripotente stamceller

I Vitro Generation af Somite derivater fra menneskelige induceret celler pluripotente stamceller

Read Article