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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
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JoVE Journal Chemistry
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

DOI:
10.3791/58744-v

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09:32 min

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April 12, 2019

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, ,
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Chapters

  • 00:04Title
  • 00:29Circular DNA Extraction
  • 02:17Assembly Solution Annealing
  • 04:53Finite Lattice Purification
  • 06:17Atomic Force Microscopy (AFM) Imaging
  • 07:58Results: Representative 1D and 2D DNA Lattice Analyses
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a detailed protocol for T4 ligation and denaturing PAGE purification of small circular DNA molecules, annealing and native PAGE analysis of circular tiles, assembling and AFM imaging of 1D and 2D DNA nanostructures, as well as agarose gel electrophoresis and centrifugation purification of finite DNA nanostructures.

Tags

Keywords: DNA NanotechnologyCircular DNADNA Motifs1D And 2D NanostructuresDNA PurificationDenaturing PAGE GelDNA AnnealingDNA AssemblyLinear DNACircular DNA StrandsDNA Lattices

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