Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Lin Sun; Naoko Kono; Hiroyuki Toh; Hanbing Xue; Kaori Sano; Tadaki Suzuki; Akira Ainai; Yasuko Orba; Junya Yamagishi; Hideki Hasegawa; Yoshimasa Takahashi; Shigeyuki Itamura; Kazuo Ohnishi

doi:10.3791/58804

Journal

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Immunology and Infection

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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

12,380 Views

•

08:51 min

•

March 15, 2019

DOI:

10.3791/58804-v

DOI:

10.3791/58804-v

•

08:51 min

March 15, 2019

•

12338 Views

Lin Sun¹, Naoko Kono², Hiroyuki Toh³, Hanbing Xue¹, Kaori Sano^4,5, Tadaki Suzuki⁴, Akira Ainai⁴, Yasuko Orba⁶, Junya Yamagishi^7,8, Hideki Hasegawa^4,5, Yoshimasa Takahashi⁹, Shigeyuki Itamura², Kazuo Ohnishi^9,10

¹Graduate School of Life and Environmental Sciences,University of Tsukuba ²Center for Influenza Virus Research,National Institute of Infectious Diseases ³School of Science and Technology,Kwansei Gakuin University ⁴Department of Pathology,National Institute of Infectious Diseases ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases,Tohoku University Graduate School of Medicine ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control,Hokkaido University ⁷Division of Collaboration and Education, Research Center for Zoonosis Control,Hokkaido University ⁸Global Station for Zoonosis Control, GI-CoRE,Hokkaido University ⁹Department of Immunology,National Institute of Infectious Diseases ¹⁰Faculty of Life and Environmental Sciences,University of Tsukuba

Summary

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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Summary

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