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JoVE Journal
Immunology and Infection

This content is Open Access.

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
 

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Article DOI: 10.3791/58804-v 08:51 min March 15th, 2019
March 15th, 2019

Chapters

Summary

Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

Transcript

Tags

Identification Mouse Human Antibody Repertoires Next-generation Sequencing Visualize Analyze B Cell Repertoires Immune Stimulation Pathological Samples Emerging Viral Infections Pathogenesis Vaccine Control Vaccine Efficacy Antibody Repertoire Dynamics PCR Conditions Antibody Gene Amplification Immunoglobulin Amplicons Tissue Dissection Cell Dispersion
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