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JoVE Journal
Immunology and Infection

This content is Open Access.

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation
 

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Article doi: 10.3791/59150
January 29th, 2019 Usage Statistics

Summary January 29th, 2019

This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.

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