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Immunology and Infection

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JoVE Journal Immunology and Infection
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A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Article DOI: 10.3791/59150-v • 09:59 min • January 29th, 2019
January 29th, 2019 •
*1, *1, 1, 1, 1, 1, 1
1Department of Nephrology, Leiden University Medical Center
* These authors contributed equally

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Summary
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This protocol describes a highly sensitive and high throughput neutrophil extracellular trap (NET) assay for the semi-automated quantification of ex vivo NET formation by immunofluorescence three-dimensional confocal microscopy. This protocol can be used to evaluate NET formation and degradation after different stimuli and can be used to study potential NET-targeted therapies.

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High-throughput Assay Neutrophil Extracellular Trap Formation NETs DNA Structures Immunofluorescence Confocal Microscopy Autoimmune Diseases ANCA-associated Vasculitis Systemic Lupus Erythematosis Therapeutics Vitro Neutrophil Isolation Peripheral Blood EDTA-coded Tubes Density Gradient
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