Three-dimensional Immunofluorescence
Three-dimensional Immunofluorescence - Related Videos
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Immunofluorescence Microscopy: Immunofluorescence Staining of Paraffin-Embedded Tissue Sections
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2023
Source: Thomas Chaffee1, Thomas S. Griffith2,3,4, and Kathryn L. Schwertfeger1,3,4 1 Department of Lab Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455 2 Department of Urology, University of Minnesota, Minneapolis, MN 55455 3 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 4 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 Pathologic analyses of tissue sections can be used to obtain a better understanding of normal tissue...
10:56
Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
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Cited by 9 •
2015
Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.
03:40
Dimensional Analysis
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2020
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units. Conversion Factors and Dimensional Analysis The unit...
01:12
Immunofluorescence Microscopy
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2023
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange. The...
04:05
An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus
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2025
Source:Matthew J Murray1, Gary R McLean2,3, Matthew B Reeves11Institute of Immunity and Transplantation, University College London.2Cellular and Molecular Immunology Research Centre, London Metropolitan University.3National Heart and Lung Institute, Imperial College London.This video demonstrates an immunofluorescence assay designed to measure the neutralization of Human Cytomegalovirus (HCMV). Virus-specific antibodies are mixed with the virus and incubated with human fibroblast cells. The...
FAQs
The microscope collects fluorescence from successive optical sections through an intact cell, tissue, or organoid. These depth-resolved images preserve the relative positions of labeled structures and can be combined into a three-dimensional reconstruction. The resulting map shows where a molecular marker occurs throughout the specimen rather than only where it appears in a single viewing plane.
Fluorescently labeled antibodies bind their intended molecular markers, producing signals that distinguish those structures from surrounding material. This binding-based contrast allows researchers to associate observed fluorescence with particular proteins or other targets. Interpretation therefore depends on relating the detected signal to the marker being localized within the preserved three-dimensional specimen.
By retaining spatial relationships across specimen depth, the method can show how immune cells are organized, where pathogens are located, and how microbes interact with host cells. It also helps visualize tissue penetration, linking molecular-marker distribution with the physical arrangement of infected or inflamed tissue.
A typical workflow begins with an intact cell, tissue, or organoid and fluorescently labeled antibodies directed toward selected molecular markers. The specimen is then examined by collecting optical sections at successive depths. Those sections are assembled into a three-dimensional view, allowing the distribution and spatial relationships of the labeled targets to be examined.
Researchers can apply the method when the location of immune cells, pathogens, or host and microbial markers matters alongside their molecular identity. It is especially relevant for examining immune-cell organization, infection-related tissue penetration, and host-microbe interactions. These observations can clarify how infection and inflammation are spatially arranged within intact biological material.
Three-dimensional distributions of molecular markers provide spatial measurements that can be used in biomarker analysis and in evaluating vaccines or antimicrobial therapies. For infection and inflammation studies, the maps can connect treatment-related changes with pathogen localization, immune organization, or tissue penetration, helping researchers assess outcomes in their original spatial context.