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Journal
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Biology
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线虫
的核苷酸和蛋白质提取联合
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Combined Nucleotide and Protein Extractions in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
线虫
的核苷酸和蛋白质提取联合
DOI:
10.3791/59178-v
•
10:37 min
•
March 17, 2019
•
Joslyn Mills
,
Erin McConnell
,
Joshua A. Leitão
,
Louis R. Lapierre
1
Department of Molecular Biology, Cell Biology and Biochemistry
,
Brown University
Chapters
00:04
Title
00:33
Sample Collection
01:31
Nucleotide and Protein Isolation
07:01
Evaluating mRNA with RT-qPCR
08:12
Results: Extractions of Different Macromolecules from the Same
C. elegans
Sample
09:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们提出了一个从同一样本中分离 RNA、DNA 和蛋白质的协议, 以减少变异, 提高重现性, 并促进解释。
Tags
Caenorhabditis Elegans
Nucleotide Extraction
Protein Extraction
MRNA
Macromolecules
Sample Variability
Reproducibility
Sample Collection
Cross-sectional Analysis
Worms
M9 Buffer
GTCP Reagent
Chloroform
RNA Isolation
Isopropanol
Ethanol
DNA Isolation
Protein Isolation
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